描述：His抗體A00186 Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags.
| 品牌 | GenScript/金斯瑞 | 貨號(hào) | A00186 |
|---|---|---|---|
| 規(guī)格 | 100ug | 供貨周期 | 一周 |
| 應(yīng)用領(lǐng)域 | 生物產(chǎn)業(yè) |
一、His抗體A00186產(chǎn)品介紹：
Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. However, since 6XHis-tag is poorly immunogenic, it needs to be conjugated to KLH or some other carrier as an immunogen. After hundreds of selection cycles, researchers at GenScript successfully isolated an antibody against His-tag.
THE™ His Tag Antibody, mAb, Mouse (subtype IgG1) has very high affinity. Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags. This means that even if the 6xHis-tag is only partially exposed, it will still be recognized and bound by this antibody.
THETM His Tag mAb is produced from mice ascites and purified by protein A affinity column. This antibody recognizes native as well as denatured forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The product reacts with fusion proteins expressed in bacteria, insect cells, and mammalian cells. THETM His Tag mAb recognizes His tags placed at N-terminal, C-terminal, and internal regions of fusion proteins.
THETM His Tag mAb can be used in Western blot analyses, Dot blot analyses, ELISA, immunofluorescent staining, and flow cytometry of cultured cells.
中文：
特異于6個(gè)His標(biāo)簽的單克隆抗體可以極大地提高幾種不同免疫分析的有效性，幫助研究人員識(shí)別、檢測(cè)和純化細(xì)菌、昆蟲細(xì)胞和哺乳動(dòng)物細(xì)胞中的多His融合蛋白。然而，由于6XHis標(biāo)簽的免疫原性較差，因此需要與KLH或其他載體結(jié)合作為免疫原。經(jīng)過數(shù)百個(gè)選擇周期后，GenScript的研究人員成功地分離出一種針對(duì)他的標(biāo)簽的抗體。
這個(gè)™ His標(biāo)記抗體mAb與小鼠（IgG1亞型）具有很高的親和力。在GenScript上進(jìn)行的測(cè)試顯示抗體也能識(shí)別4xHis和5xHis標(biāo)簽。這意味著即使6xHis標(biāo)簽僅部分暴露，它仍將被該抗體識(shí)別和結(jié)合。
THTM His標(biāo)記單克隆抗體由小鼠腹水制備，并通過蛋白A親和柱純化。該抗體識(shí)別天然和變性形式的合成多His和多His標(biāo)記的融合蛋白。該產(chǎn)物與細(xì)菌、昆蟲細(xì)胞和哺乳動(dòng)物細(xì)胞中表達(dá)的融合蛋白反應(yīng)。THTM-His標(biāo)簽單克隆抗體識(shí)別位于融合蛋白N端、C端和內(nèi)部區(qū)域的His標(biāo)簽。
THTM-His標(biāo)記單抗可用于培養(yǎng)細(xì)胞的Western印跡分析、斑點(diǎn)印跡分析、ELISA、免疫熒光染色和流式細(xì)胞術(shù)。
二、His抗體A00186產(chǎn)品特性：
| Host Species | Mouse |
| Conjugation | Unconjugated |
| Immunogen | A synthetic peptide HHHHHH coupled to KLH |
| Fusion Partner | Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells |
| Purification | Protein A affinity column |
| Subclass | IgG1, k |
| Clone ID | 6G2A9 |
| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
| Specificity | THE™ His Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins. |
| Storage | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Note | GenScript can offer this product according to your requirement, including product size, buffer components,etc. |
三、產(chǎn)品應(yīng)用：
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
四、公司介紹：
漠寶（廈門）生物科技有限公司是一家致力于生命科學(xué)和生物技術(shù)領(lǐng)域的企業(yè)，由廈門大學(xué)研究生創(chuàng)辦。公司主要專注于生命科學(xué)科研試劑、耗材、儀器等科研用品的開發(fā)與銷售。公司主要代理歐美著名生物技術(shù)公司的產(chǎn)品，能為生命科學(xué)多研究領(lǐng)域的科研人員提供全面的產(chǎn)品。我們相信經(jīng)過幾年的努力，漠寶生物一定能成為分子生物學(xué)、細(xì)胞生物學(xué)、免疫學(xué)等領(lǐng)域科研產(chǎn)品的供應(yīng)商。
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展望：公司將借助于廈門大學(xué)細(xì)胞應(yīng)激生物學(xué)國(guó)家重點(diǎn)實(shí)驗(yàn)室的人才和技術(shù)優(yōu)勢(shì)，致力于研發(fā)、生產(chǎn)出品質(zhì)*的生命科學(xué)研究中用到的試劑、抗體、試劑盒等，力爭(zhēng)在不久的將來將漠寶打造為生命科學(xué)領(lǐng)域的。
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